Loss of IkBa-Mediated Control over Nuclear Import and DNA Binding Enables Oncogenic Activation of c-Rel

Nuclear Localization of IkBa Is Mediated by the Second Ankyrin Repeat: the IkBa Ankyrin Repeats Define a Novel Class of cis-Acting Nuclear Import Sequences

The ability of the IkBa protein to sequester dimeric NF-kB/Rel proteins in the cytoplasm provides an effective mechanism for regulating the potent transcriptional activation properties of NF-kB/Rel family members. IkBa can also act in the nucleus as a postinduction repressor of NF-kB/Rel proteins. The mechanism by which IkBa enters the nucleus is not known, as IkBa lacks a discernible classical...

Activation of the Transcriptional Function of the NF-kB Protein c-Rel by O-GlcNAc Glycosylation

D ow nladed fr The transcription factor nuclear factor kB (NF-kB) rapidly reprograms gene expression in response to various stimuli, and its activity is regulated by several posttranslational modifications, including phosphorylation, methylation, and acetylation. The addition of O-linked b-N-acetylglucosamine (a process known as O-GlcNAcylation) is an abundant posttranslational modification tha...

The Dorsal Rel homology domain plays an active role in transcriptional regulation.

The Dorsal morphogen directs formation of the Drosophila dorsoventral axis by both activating and repressing transcription. It contains an N-terminal Rel homology domain (RHD), which is responsible for DNA binding and regulated nuclear import, and a C-terminal domain (CTD) that contains activation and repression motifs. To determine if the RHD has a direct role in transcriptional control, we an...

CpG DNA Rescue from Anti-IgM-Induced WEHI-231 B Lymphoma Apoptosis via Modulation of IkBa and IkBb and Sustained Activation of Nuclear Factor-kB/c-Rel

Nuclear Import of IkBa Is Accomplished by a Ran-Independent Transport Pathway

The inhibitor of kappa B alpha (IkBa) protein is able to shuttle between the cytoplasm and the nucleus. We have utilized a combination of in vivo and in vitro approaches to provide mechanistic insight into nucleocytoplasmic shuttling by IkBa. IkBa contains multiple functional domains that contribute to shuttling of IkBa between the cytoplasm and the nucleus. Nuclear import of IkBa is mediated b...